Course Name: Analytical chemistry experiment（Basic chemistry experiment II）
Pre-requisite: Analytical chemistry
Lecture Time: 56 class hours
This course is a compulsory professional course for undergraduates majoring in chemistry, and is composed of two parts: chemical analysis and instrumental analysis. It is both an independent course and closely matched with analytical chemistry theory course. After learning this course, students are expected to master the basic knowledge, basic operations, basic skills, typical analytical methods and experimental data processing methods of analytical chemical experiment. Students should establish the concepts of "quantitative", "error", "Deviation" and “significant figure”, understand and grasp the main factors and key links that affect test results, and also make reasonable selection of experimental conditions and experimental instruments to ensure the reliability of quantitative results. Through experiments, students enlarge understandings of the relevant theories and are able to apply the theoretical and experimental knowledge to guide the design and operation of the experiment. Deepen the understanding of the basic principles of instrumental analysis methods to master the applied ranges and main analysis objects of various instrumental analysis methods.Master the basic operation methods of various analytical instruments and the processing methods of the experimental data, especially the influence of main operating parameters on the analysis results. Improve the ability to analyze and solve practical problems independently and think overall. The undergraduate students will cultivate the consciousness of innovation and exploration. Meanwhile, they develop rigorous scientific styles and good experimental accomplishment, laying a solid foundation for future work.
Examination method：Experiment report
Topics and Schedule
Experiment 1 Quantitative analysis instrument and basic operation
Learn to master the use of electronic analytical balance and precautions, learn how to use the acidity meter. Learn to master the micro titration apparatus and operation, to understand the significance of the calibration of capacity instrument.
Experiment 2 Acid-Base titration experiment
Learn to master the titration procedures, observe the end points and readings. Learn the principles and operations of back titration method by determining acetylsalicylic acid in aspirin tablets. Master data processing and analysis methods.
Experiment 3 Complexation titration experiment
The basic principle of Complexation Titration was enhanced by the continuous determination of Pb2+ and Bi3+ content in lead and bismuth alloy. To understand the significance of controlling the acidity of the solution in the complexation titration. To understand the judgement of use and finish of xylenol orange indicator. To master data processing and analysis.
Experiment 4 Determination of cadmium in water by Flame Atomic Absorption Spectrometry
Operation technique of flame atomic absorption spectrometer, optimizations of flame conditions. To draw working curves and make quantitative analyses of samples.
Experiment 5 Ultraviolet absorption spectrum analysis and data processing of Potassium Permanganate
Drawing the ultraviolet absorption curve of Potassium Permanganate in different solvents and different concentrations, and understanding the relationship between UV absorption spectrum, concentration, molecular structure and solvent.
Experiment 6 Determine the fluorescence spectra of dichlorofluorescein
Drawing the fluorescence emission spectra of dichlorofluorescein, analyzing and determining the fluorescence emission peak of dichlorofluorescein, determining the maximum emission wavelength of dichlorofluorescein, and measuring its maximum excitation wavelength.
Experiment 7 Determination of infrared transmission spectroscopy for different samples
Methods for preparing solid, liquid and gas samples, sample detection, and data processing.
Experiment 8 Determination of inorganic and organic compounds by Raman spectroscopy
Basic method for Raman spectroscopy detection of samples, data processing, such as baseline correction, map analysis, etc.
Experiment 9 Ion selective electrode method for the determination of F- in natural water
Measurement method of ion selective electrode; drawing of standard curve; calculation of the actual content of F- in water.
Experiment 10 Quantitative analysis by gas chromatography
The operation method of gas chromatography. With benzene as reference substance, determine the quantitative correction factor of hexane, cyclohexane and toluene. Determine the content of hexane and cyclohexane in the mixture according to chromatogram. Determining the content of toluene in the mixture by external standard method.
Experiment 11 Determining the content of caffeine in beverages by high performance liquid chromatography
Operation method of high performance liquid chromatograph;the concentration of caffeine in beverages was calculated with the peak area of the reference substance and the corresponding drawing working curve.
Experiment 12 Capillary zone electrophoresis separates Isomers of nitro phenol
Operation method of capillary electrophoresis apparatus; calculating the separation degree between the components according to the separation graph; drawing the relationship graph between electric voltage and electroosmotic velocity; drawing the standard curve and determining the concentration of the unknown compound.
Experiment 13 Mass spectrometry for the analysis of unknown structures
Grasping the basic principles of liquid mass spectrometry; grasping the operation method of liquid chromatography-mass spectrometer; grasping the method of identifying the structure of unknown compounds based on ion fragments.
 Wuhan University, Analytical Chemistry Experiment (I), Fifth Edition, Higher Education Press, 2006
 Laboratory center of chemistry and molecular science (Wuhan University), Instrumental Analysis Experiment, First Edition, Wuhan University Press, Wuhan, 2005
 Wuhan University, Analytical Chemistry（Ⅰ、Ⅱ）, Fifth Edition, Higher Education Press, 2006
 Chonghui Peng, Jianzhang Feng, Tinyu Zhang etc., Analytical chemistry: A Concise Course in Quantitative Chemical Analysis, Third Edition, Peking University Press, 2009
 College of Chemistry and Molecular Engineering（Peking University）,Basic Analytical Chemistry Experiment, Third Edition, Peking University Press, 2010
 Peirong Chen, Jinghong Li, Bo Deng,Modern Instrumental Analysis Experiment and Technology, Second Edition, Tsinghua University Press, Beijing, 2006